Fluorescence Anisotropy Imaging Microscopy
When excited by laser light, structured, fluorescence-labeled proteins show a directed emission depending on the polarizing direction of the laser. This property, known as anisoptropy, can be investigated very easily with a confocal system and the lasers used with it.


Aktinfilamente im Auge von Drosophila melanogaster
Actin filaments labelled with AlexaFluor488-phalloidin in the Drosophila eye.
Each photoreceptor cell has an array of densely packed microvilli; each microvillus contains a single actin filament in its centre. These microvillar arrays (rhabdomeres) are seen as oval structures in the image.
Specimen: Otto Baumann, University of Potsdam, Department of
Biochemistry and Biology, Germany
Aktinfilamente im Auge von Drosophila melanogaster
Actin filaments labelled with AlexaFluor488-phalloidin in the Drosophila eye.
Image showing the result of the evaluation of the Anisotropy of the specimen.
The actin filaments of the displayed rhabdomeres are similarily oriented.
Specimen: Otto Baumann, University of Potsdam, Department of
Biochemistry and Biology, Germany
enlarge
Actin filaments labelled with AlexaFluor488-phalloidin in the Drosophila eye.
Each photoreceptor cell has an array of densely packed microvilli; each microvillus contains a single actin filament in its centre. These microvillar arrays (rhabdomeres) are seen as oval structures in the image.
Specimen: Otto Baumann, University of Potsdam, Department of
Biochemistry and Biology, Germany
enlarge
Actin filaments labelled with AlexaFluor488-phalloidin in the Drosophila eye.
Image showing the result of the evaluation of the Anisotropy of the specimen.
The actin filaments of the displayed rhabdomeres are similarily oriented.
Specimen: Otto Baumann, University of Potsdam, Department of
Biochemistry and Biology, Germany
Actin filaments labelled with
AlexaFluor488-phalloidin in the Drosophila eye. 		Image showing the result of the evaluation of the Anisotropy of the specimen.
Anisotropy imaging is now offered with the LSM 710 / LSM 780 Laser Scanning Microscope as an option. Special filters ensure a very high extinction ratio of the respective other polarizing direction of the emission and, thus, images of exceptional contrast and information content.

The result of the anisotropy determination is displayed in a separate image. The method permits changes of the structural arrangement to be tracked over time. The spectral recording of the emission signal makes it possible to simultaneously detect several fluorescence signals as a function of their polarization.

Anisotropy imaging is another imaging method permitting you to utilize, in addition to intensity differences and the spectrum, another parameter of the emission light for the investigation of proteins.

The filters required for the method can be supplied with, or retrofitted to, any LSM 710 / LSM 780.

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